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Product Info Summary
| SKU: | M01394 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Iba1 AIF1 Monoclonal Antibody
View all AIF-1/Iba1 Antibodies
SKU/Catalog Number
M01394
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Iba1 AIF1 Monoclonal Antibody catalog # M01394. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Iba1 AIF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEBB-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Iba1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01394 is reactive to AIF1 in Human, Mouse, Rat
Applications
M01394 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
120-140 kDa
Calculated molecular weight
16.703kDa
Background of AIF-1/Iba1
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:100-1:500
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of Iba1 expression in U937 cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Iba1 antibody.
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Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-Iba1 antibody.
Protein Target Info & Infographic
Gene/Protein Information For AIF1 (Source: Uniprot.org, NCBI)
Gene Name
AIF1
Full Name
Allograft inflammatory factor 1
Weight
16.703kDa
Alternative Names
AIF1; AIF-1; AIF-1IRT1; allograft inflammatory factor 1; G1; IBA1; IBA1Em:AF129756.17; IbaI; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT1; IRT-1; Protein G1 AIF1 AIF-1, IBA1, IRT-1, IRT1 allograft inflammatory factor 1 allograft inflammatory factor 1|interferon gamma responsive transcript|ionized calcium-binding adapter molecule 1|protein G1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AIF1, check out the AIF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AIF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Iba1 AIF1 Monoclonal Antibody (M01394)
Hello CJ!
M01394 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M01394 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:1000
Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M01394 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:500
Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
M01394 usage in article: APP:IHC, SAMPLE:BRAIN SECTION, DILUTION:1:400
Mohamed MY,Masocha W. Indomethacin augments lipopolysaccharide-induced expression of inflammatory molecules in the mouse brain. PeerJ.2020 Nov 18;8:e10391.doi:10.7717/peerj.10391.PMID:33240677;PMCID:PMC7680052.
Species: Mouse
M01394 usage in article: APP:WB, SAMPLE:BRAIN HOMOGENATES, DILUTION:1:50
Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway
Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture
Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocyte activation in male mice
Re-infection of the prion from the scrapie?infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-Iba1 AIF1 Monoclonal Antibody
Question
Is there a protocol for M01394 in which it was tested positive?
Verified customer
Asked: 2021-02-18
Answer
We recommend the use of the Anti-Iba1 AIF1 Monoclonal Antibody (M01394) in methanol fixed IHC stainings of mouse brain cryosections. The application was customer reported.
Boster Scientific Support
Answered: 2021-02-22
Question
Here is the WB image, lot number and protocol we used for lymphocyte using anti-Iba1 Monoclonal antibody M01394. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-24
Question
We are currently using anti-Iba1 Monoclonal antibody M01394 for mouse tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-Iba1 Monoclonal antibody (M01394) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
I am interested in to test anti-Iba1 Monoclonal antibody M01394 on human lymphocyte for research purposes, then I may be interested in using anti-Iba1 Monoclonal antibody M01394 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The products we sell, including anti-Iba1 Monoclonal antibody M01394, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-05
Question
We have tried in the past anti-Iba1 Monoclonal antibody for WB on liver in a previous project. I am using rat, and We are going to use the antibody for IHC next. My question regards examining liver as well as erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
I looked at the website and datasheets of our anti-Iba1 Monoclonal antibody and I see that M01394 has been tested on rat in both WB and IHC. Thus M01394 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-04
Question
My boss were satisfied with the WB result of your anti-Iba1 Monoclonal antibody. However we have been able to see positive staining in erythroleukemia cytoskeleton using this antibody. Is that expected? Could you tell me where is AIF1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
From what I have seen in literature, erythroleukemia does express AIF1. Generally AIF1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-Iba1 Monoclonal antibody for IF for human lymphocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphocyte identification?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can see on the product datasheet, M01394 anti-Iba1 Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-25
Question
Will anti-Iba1 Monoclonal antibody M01394 work for IF with lymphocyte?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
According to the expression profile of lymphocyte, AIF1 is highly expressed in lymphocyte. So, it is likely that anti-Iba1 Monoclonal antibody M01394 will work for IF with lymphocyte.
Boster Scientific Support
Answered: 2019-07-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-Iba1 Monoclonal antibody M01394. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-Iba1 Monoclonal antibody M01394 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-21
Question
We need using your anti-Iba1 Monoclonal antibody for positive regulation of protein phosphorylation studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thank you for your inquiry. This M01394 anti-Iba1 Monoclonal antibody is tested on mouse thymus tissue, brain tissue, rat thymus tissue, spleen tissue. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this M01394 anti-Iba1 Monoclonal antibody reactive to the isotypes of AIF1?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The immunogen of M01394 anti-Iba1 Monoclonal antibody is A synthesized peptide derived from human Iba1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-09
Question
We have observed staining in mouse liver. Are there any suggestions? Is anti-Iba1 Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
From what I have seen in literature liver does express AIF1. From what I have seen in Uniprot.org, AIF1 is expressed in metanephric glomerulus, lymphocyte, glial tumor, prostate, erythroleukemia, liver, among other tissues. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-02-19
Question
Is a blocking peptide available for product anti-Iba1 Monoclonal antibody (M01394)?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
We do provide the blocking peptide for product anti-Iba1 Monoclonal antibody (M01394). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-09
Question
Is there a BSA free version of anti-Iba1 Monoclonal antibody M01394 available?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Iba1 Monoclonal antibody M01394 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-24
Question
Can you help my question with product M01394, anti-Iba1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Jha
Verified customer
Asked: 2017-03-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01394 anti-Iba1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-06
Question
Will M01394 anti-Iba1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Carter
Verified customer
Asked: 2013-12-03
Answer
It shows on the product datasheet, M01394 anti-Iba1 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-03
